Transfecting

January 26, 2008

The cells were placed in AB free medium. One of the plates just had the medium changed. One plate was treated with a plasmid that only contained the drug resistance protein. The last plate was treated with a plasmid containing the drug resistance protein and the CPAK2 protein. All three plates continue to be incubated.

January 28, 2008

We treated all the cells plates with the drug G-418.  This drug will kill all the cells that do not present the drug resistance protein.  This will tell us which cells present the CPAK2 protein.

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