So today i went into the lab and got all excited b/c i thought that i saw a colony of cells that had grown up. Later, I discovered that it was no more than a spot of dirt on the bottom of my petri dish. All the tissue culture cells were dead too. Sad day! So now we have to go back and start all over again. This entails that the DNA be re-purified, the cells be transvected, and left to grow again. This time we hope that it works.

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