So today i went into the lab and got all excited b/c i thought that i saw a colony of cells that had grown up. Later, I discovered that it was no more than a spot of dirt on the bottom of my petri dish. All the tissue culture cells were dead too. Sad day! So [...]
January 26, 2008
The cells were placed in AB free medium. One of the plates just had the medium changed. One plate was treated with a plasmid that only contained the drug resistance protein. The last plate was treated with a plasmid containing the drug resistance protein and the CPAK2 protein. All three plates continue to [...]
January 25, 2008
So, the cells grew a little too well. There were several dead cells found when we took the plate out to split cells. On this day, the goal was to make 3 new plates with about 100,000 cells each. To get a rough estimate of how many cells were in the plate, we [...]
Today was the first day that I have ever worked in a real lab. It was amazing. It was just a small step in the direction my major will take me. This semester I am going to be working with tissue cells. The goal is to change the DNA of the cells to make them produce a specific protein and a [...]
